PrimePCR™ Probe Assay: MYB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MYB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MYB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051043
Assay Design:   exonic
Chromosome Location:   6:135511286-135511420question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000341911 ENST00000442647 ENST00000367814 ENST00000527615 ENST00000420123 ENST00000525369 ENST00000528774 ENST00000534121 ENST00000534044 ENST00000533624 ENST00000430686 ENST00000316528

Gene Information

This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains an N-terminal DNA-binding domain a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jun 2009]

Gene Symbol:   MYB
Gene Name:   v-myb myeloblastosis viral oncogene homolog (avian)
Aliases:   Cmyb, c-myb, c-myb_CDS, efg
RefSeq:   NC_000006.11 NT_025741.15 NG_012330.1
Ensembl:   ENSG00000118513
Entrez:   4602
Chromosome Mapping:   6q22-q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.610000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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