PrimePCR™ Probe Assay: TFAP2A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TFAP2A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TFAP2A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052015
Assay Design:   exonic
Chromosome Location:   6:10404837-10404938question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000379613 ENST00000319516 ENST00000379608 ENST00000482890 ENST00000466073 ENST00000475264 ENST00000498450 ENST00000379604 ENST00000366910 ENST00000407981 ENST00000373869 ENST00000288065 ENST00000373866

Gene Information

The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Dec 2009]

Gene Symbol:   TFAP2A
Gene Name:   transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
Aliases:   AP-2, AP-2alpha, AP2TF, BOFS, FLJ51761, TFAP2
RefSeq:   NC_000006.11 NT_007592.15 NG_016151.1
Ensembl:   ENSG00000137203
Entrez:   7020
Chromosome Mapping:   6p24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.250000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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