PrimePCR™ Probe Assay: POMC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: POMC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POMC, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041643
Assay Design:   Intron-spanning
Chromosome Location:   2:25384567-25388518question
Amplicon Length:   190
Splice Variants Targeted:   ENST00000449297 ENST00000370754 ENST00000462484 ENST00000460843 ENST00000334856 ENST00000554971 ENST00000381578 ENST00000538382 ENST00000405623 ENST00000264708 ENST00000395826 ENST00000449220 ENST00000380794 ENST00000529308 ENST00000526425 ENST00000361735

Gene Information

This gene encodes a polypeptide hormone precursor that undergoes extensive tissue-specific post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and depending on tissue type and the available convertases processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin essential for normal steroidogenesis and the maintenance of normal adrenal weight and lipotropin beta are the major end products. In other tissues including the hypothalamus placenta and epithelium all cleavage sites may be used giving rise to peptides with roles in pain and energy homeostasis melanocyte stimulation and immune modulation. These include several distinct melanotropins lipotropins and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity adrenal insufficiency and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   POMC
Gene Name:   proopiomelanocortin
Aliases:   ACTH, CLIP, LPH, MSH, NPP, POC
RefSeq:   NC_000002.11 NT_022184.15 NG_008997.1
Ensembl:   ENSG00000115138
Entrez:   5443
Chromosome Mapping:   2p23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.000000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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