PrimePCR™ Probe Assay: PTPRC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PTPRC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PTPRC, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041630
Assay Design:   exonic
Chromosome Location:   1:198608356-198608454question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000367379 ENST00000530727 ENST00000442510 ENST00000367367 ENST00000348564 ENST00000367364 ENST00000413409 ENST00000418674 ENST00000367376 ENST00000352140 ENST00000594404 ENST00000598951 ENST00000573298 ENST00000575923 ENST00000573679 ENST00000576833 ENST00000573477 ENST00000570609 ENST00000571847 ENST00000573971

Gene Information

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitotic cycle and oncogenic transformation. This PTP contains an extracellular domain a single transmembrane segment and two tandem intracytoplasmic catalytic domains and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene which encode distinct isoforms have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   PTPRC
Gene Name:   protein tyrosine phosphatase, receptor type, C
Aliases:   B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200
RefSeq:   NC_000001.10 NG_007730.1 NT_004487.19 NW_003315907.1
Ensembl:   ENSG00000081237
Entrez:   5788
Chromosome Mapping:   1q31-q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 37.220000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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