PrimePCR™ Probe Assay: SELP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SELP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SELP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028101
Assay Design:   Intron-spanning
Chromosome Location:   1:169582829-169586317question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000367795 ENST00000446728 ENST00000367790 ENST00000426706 ENST00000367789 ENST00000367788 ENST00000367786 ENST00000458599 ENST00000263686 ENST00000544572 ENST00000367793 ENST00000367794 ENST00000367792 ENST00000367791

Gene Information

This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq Jul 2008]

Gene Symbol:   SELP
Gene Name:   selectin P (granule membrane protein 140kDa, antigen CD62)
Aliases:   CD62, CD62P, FLJ45155, GMP140, GRMP, LECAM3, PADGEM, PSEL
RefSeq:   NC_000001.10 NG_011806.1 NG_012125.1 NT_004487.19
Ensembl:   ENSG00000174175
Entrez:   6403
Chromosome Mapping:   1q22-q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 34.920000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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