PrimePCR™ Probe Assay: Nme2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Nme2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Nme2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCIP0043027
Assay Design:   Intron-spanning
Chromosome Location:   11:93950016-93952802question
Amplicon Length:   116
Splice Variants Targeted:   ENSMUST00000146271 ENSMUST00000021217 ENSMUST00000072566 ENSMUST00000170303 ENSMUST00000135884 ENSMUST00000021220

Gene Information

Description Not Available

Gene Symbol:   Nme2
Gene Name:   NME/NM23 nucleoside diphosphate kinase 2
Aliases:   NM23-H2, NM23B, nm23-M2
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000020857
Entrez:   18103
Chromosome Mapping:   11|11 C

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 38.150000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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