PrimePCR™ Probe Assay: PIK3CG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PIK3CG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PIK3CG, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029621
Assay Design:   Intron-spanning
Chromosome Location:   7:106520082-106523515question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000440650 ENST00000496166 ENST00000473541 ENST00000359195

Gene Information

This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals including those elicited by E-cadherin-mediated cell-cell adhesion which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. [provided by RefSeq Jul 2008]

Gene Symbol:   PIK3CG
Gene Name:   phosphoinositide-3-kinase, catalytic, gamma polypeptide
Aliases:   PI3CG, PI3K, PI3Kgamma, PIK3
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000105851
Entrez:   5294
Chromosome Mapping:   7q22.3

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