PrimePCR™ Probe Assay: RAP1GAP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RAP1GAP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAP1GAP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024849
Assay Design:   Exonic
Chromosome Location:   1:21940121-21940503question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000374765 ENST00000374763 ENST00000359708 ENST00000374757 ENST00000290101 ENST00000374761 ENST00000542643 ENST00000374758

Gene Information

This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene RAP1GAP promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins guanine nucleotide exchange factors (GEFs) act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general ras subfamily proteins such as RAP1 play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation adhesion differentiation and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq Aug 2011]

Gene Symbol:   RAP1GAP
Gene Name:   RAP1 GTPase activating protein
Aliases:   KIAA0474, RAP1GA1, RAP1GAP1, RAP1GAPII, RAPGAP
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000076864
Entrez:   5909
Chromosome Mapping:   1p36.1-p35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996500
y-intercept 38.010000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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