PrimePCR™ Probe Assay: MEF2C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MEF2C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MEF2C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028437
Assay Design:   Intron-spanning
Chromosome Location:   5:88024397-88025124question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000340208 ENST00000424173 ENST00000514028 ENST00000437473 ENST00000510942 ENST00000506554 ENST00000508569 ENST00000514015 ENST00000504921 ENST00000539796

Gene Information

This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins which play a role in myogenesis. The encoded protein MEF2 polypeptide C has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation stereotypic movements epilepsy and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq Jul 2010]

Gene Symbol:   MEF2C
Gene Name:   myocyte enhancer factor 2C
Aliases:   C5DELq14.3; DEL5q14.3
RefSeq:   NC_000005.9 NG_023427.1 NT_006713.15
Ensembl:   ENSG00000081189
Entrez:   4208
Chromosome Mapping:   5q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.050000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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