PrimePCR™ Probe Assay: ANXA2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ANXA2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ANXA2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041682
Assay Design:   Intron-spanning
Chromosome Location:   15:60653204-60656703question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000396024 ENST00000451270 ENST00000332680 ENST00000421017 ENST00000559176 ENST00000558985 ENST00000560466 ENST00000559818 ENST00000560367 ENST00000558998 ENST00000560389 ENST00000560165 ENST00000559113 ENST00000557906 ENST00000559725 ENST00000559350 ENST00000558558 ENST00000558986 ENST00000559467 ENST00000559956 ENST00000559780 ENST00000560468 ENST00000369784 ENST00000602849 ENST00000487146

Gene Information

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4 9 and 10 respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ANXA2
Gene Name:   annexin A2
Aliases:   ANX2, ANX2L4, CAL1H, LIP2, LPC2, LPC2D, P36, PAP-IV
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000182718
Entrez:   302
Chromosome Mapping:   15q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.030000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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