PrimePCR™ Probe Assay: Txnrd1, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Txnrd1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Txnrd1, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0053175
Assay Design:   exonic
Chromosome Location:   10:82896708-82896837question
Amplicon Length:   100
Splice Variants Targeted:   ENSMUST00000020484

Gene Information

This gene encodes a member of the family of pyridine nucleotide-disulfide oxidoreductases. This protein is a flavoenzyme which uses NADPH for reduction of thioredoxins as well as other protein and nonprotein substrates and plays a role in protection against oxidative stress. It contains a selenocysteine (Sec) residue which is essential for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure the sec insertion sequence (SECIS) that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   Txnrd1
Gene Name:   Thioredoxin reductase 1
Aliases:   TR, TR1, TrxR1
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000020250
Entrez:   50493
Chromosome Mapping:   10 C1|10

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