PrimePCR™ Probe Assay: TUBB, Human

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: TUBB, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: TUBB, Human
Reaction: 200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qHsaCEP0039969
Assay Design:		exonic
Chromosome Location:		6:30691959-30692107question
Amplicon Length:		119
Splice Variants Targeted:		ENST00000331320 ENST00000406191 ENST00000405646 ENST00000434050 ENST00000340384 ENST00000317702 ENST00000591208 ENST00000327892 ENST00000330914 ENST00000396389 ENST00000396384 ENST00000435534 ENST00000436628 ENST00000437490 ENST00000426754 ENST00000425102 ENST00000549902 ENST00000549230 ENST00000421473 ENST00000413547 ENST00000419551 ENST00000454620 ENST00000549614 ENST00000548314 ENST00000432462 ENST00000427480 ENST00000424734 ENST00000420618 ENST00000549654 ENST00000552725 ENST00000422650 ENST

Gene Information

Description Not Available

Gene Symbol:		TUBB
Gene Name:		tubulin, beta
Aliases:		M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB1, TUBB5
RefSeq:		NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:		ENSG00000196230
Entrez:		203068
Chromosome Mapping:		6p21.33

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.993600
y-intercept	36.360000
Efficiency	91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10000088666	Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G	Click to download
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