PrimePCR™ Probe Assay: SEMA3A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SEMA3A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SEMA3A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041232
Assay Design:   exonic
Chromosome Location:   7:83823896-83823989question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000265362 ENST00000436949 ENST00000420047 ENST00000448879 ENST00000424555

Gene Information

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent inhibiting axonal outgrowth or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq Jul 2008]

Gene Symbol:   SEMA3A
Gene Name:   sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Aliases:   Hsema-I, Hsema-III, MGC133243, SEMA1, SEMAD, SEMAIII, SEMAL, SemD, coll-1
RefSeq:   NC_000007.13 NG_011489.1 NT_007933.15
Ensembl:   ENSG00000075213
Entrez:   10371
Chromosome Mapping:   7p12.1

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