PrimePCR™ Probe Assay: NFYA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NFYA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NFYA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049133
Assay Design:   exonic
Chromosome Location:   6:41059267-41059380question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000341376 ENST00000353205

Gene Information

The protein encoded by this gene is one subunit of a trimeric complex forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit suggesting a role as the regulatory subunit. In addition there is evidence of post-transcriptional regulation in this gene product either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain with clear tissue-specific preferences for the two isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   NFYA
Gene Name:   nuclear transcription factor Y, alpha
Aliases:   CBF-A, CBF-B, FLJ11236, HAP2, NF-YA
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000001167
Entrez:   4800
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.570000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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