PrimePCR™ Probe Assay: H1F0, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: H1F0, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: H1F0, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055276
Assay Design:   exonic
Chromosome Location:   22:38202145-38202293question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000340857

Gene Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A H2B H3 and H4). The chromatin fiber is further compacted through the interaction of a linker histone H1 with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq Jul 2008]

Gene Symbol:   H1F0
Gene Name:   H1 histone family, member 0
Aliases:   H10, H1FV, MGC5241
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000189060
Entrez:   3005
Chromosome Mapping:   22q13.1

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