PrimePCR™ Probe Assay: VCL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: VCL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VCL, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0032822
Assay Design:   Intron-spanning
Chromosome Location:   10:75863626-75864902question
Amplicon Length:   125
Splice Variants Targeted:   ENST00000372755 ENST00000436396 ENST00000211998 ENST00000537043 ENST00000415462

Gene Information

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene but the biological validity of some variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   VCL
Gene Name:   vinculin
Aliases:   CMD1W, CMH15, MVCL
RefSeq:   NC_000010.10 NT_030059.13 NG_008868.1
Ensembl:   ENSG00000035403
Entrez:   7414
Chromosome Mapping:   10q22.2

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