PrimePCR™ Probe Assay: Sp3, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Sp3, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Sp3, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0054504
Assay Design:   exonic
Chromosome Location:   2:72970904-72971050question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000102689 ENSMUST00000066003

Gene Information

This gene product belongs to a family of Sp1 related transcription factors which regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains and has been reported to function as a bifunctional transcription factor that either stimulates or represses transcription of numerous genes. Alternative splicing results in transcript variants encoding different isoforms and one variant initiates translation from a non-AUG (AUA) codon. [provided by RefSeq Jul 2008]

Gene Symbol:   Sp3
Gene Name:   Trans-acting transcription factor 3
Aliases:   D130027J01Rik
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000027109
Entrez:   20687
Chromosome Mapping:   2 C3|2

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