PrimePCR™ Probe Assay: FUCA2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $270.00
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PrimePCR™ PreAmp for Probe Assay: FUCA2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FUCA2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026384
Assay Design:   Intron-spanning
Chromosome Location:   6:143828506-143832629question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000002165 ENST00000367585 ENST00000438118

Gene Information

This gene encodes a plasma alpha-L-fucosidase which represents 10-20% of the total cellular fucosidase activity. The protein is a member of the glycosyl hydrolase 29 family and catalyzes the hydrolysis of the alpha-16-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. This enzyme is essential for Helicobacter pylori adhesion to human gastric cancer cells. [provided by RefSeq Aug 2010]

Gene Symbol:   FUCA2
Gene Name:   fucosidase, alpha-L- 2, plasma
Aliases:   MGC1314, dJ20N2.5
RefSeq:   NC_000006.11 NG_027676.1 NT_025741.15
Ensembl:   ENSG00000001036
Entrez:   2519
Chromosome Mapping:   6q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.770000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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