PrimePCR™ SYBR® Green Assay: APAF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051145

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: APAF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: APAF1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043883
Assay Design:   exonic
Chromosome Location:   12:99042458-99042575question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000551964 ENST00000359972 ENST00000547743 ENST00000552268 ENST00000550527 ENST00000547045 ENST00000549007 ENST00000357310 ENST00000339433 ENST00000333991

Gene Information

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain a caspase recruitment domain (CARD) and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein releasing its mature activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   APAF1
Gene Name:   apoptotic peptidase activating factor 1
Aliases:   APAF-1, CED4, DKFZp781B1145
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000120868
Entrez:   317
Chromosome Mapping:   12q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.110000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download