PrimePCR™ Probe Assay: CASP8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CASP8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CASP8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024229
Assay Design:   Exonic
Chromosome Location:   2:202131251-202131399question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000392263 ENST00000432109 ENST00000264275 ENST00000440732 ENST00000392258 ENST00000447616 ENST00000358485 ENST00000413726 ENST00000323492 ENST00000429881 ENST00000264274 ENST00000392261 ENST00000392259 ENST00000392266

Gene Information

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain a large protease subunit and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described although not all variants have had their full-length sequences determined. [provided by RefSeq Jul 2008]

Gene Symbol:   CASP8
Gene Name:   caspase 8, apoptosis-related cysteine peptidase
Aliases:   ALPS2B, CAP4, Casp-8, FLICE, FLJ17672, MACH, MCH5, MGC78473
RefSeq:   NC_000002.11 NT_005403.17 NG_007497.1
Ensembl:   ENSG00000064012
Entrez:   841
Chromosome Mapping:   2q33-q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.320000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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