PrimePCR™ Probe Assay: HLA-DMA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HLA-DMA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HLA-DMA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040016
Assay Design:   exonic
Chromosome Location:   6:32918489-32920727question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000383227 ENST00000383230 ENST00000412394 ENST00000418042 ENST00000293677 ENST00000452330 ENST00000434337 ENST00000433630 ENST00000437285 ENST00000319888 ENST00000576251 ENST00000574538 ENST00000391713 ENST00000592355 ENST00000587340 ENST00000420931 ENST00000439054 ENST00000456486 ENST00000441171 ENST00000419041 ENST00000441375 ENST00000416806 ENST00000419231 ENST00000451148 ENST00000453490 ENST00000422196 ENST00000416297 ENST00000452434 ENST00000450601 ENST00000454298 ENST00000458066 ENST

Gene Information

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB) both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes dendritic cells macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide exons 2 and 3 encode the two extracellular domains exon 4 encodes the transmembrane domain and the cytoplasmic tail. [provided by RefSeq Jul 2008]

Gene Symbol:   HLA-DMA
Gene Name:   major histocompatibility complex, class II, DM alpha
Aliases:   D6S222E, DMA, HLADM, RING6
RefSeq:   NC_000006.11 NG_012006.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204257
Entrez:   3108
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.340000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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