PrimePCR™ Probe Assay: ESR2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ESR2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ESR2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052206
Assay Design:   exonic
Chromosome Location:   14:64749484-64749600question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000556275 ENST00000554572 ENST00000358599 ENST00000555278 ENST00000553796 ENST00000557772 ENST00000341099 ENST00000542956 ENST00000353772 ENST00000357782 ENST00000267525

Gene Information

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus cytoplasm and mitochondria. Upon binding to 17beta-estradiol or related ligands the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   ESR2
Gene Name:   estrogen receptor 2 (ER beta)
Aliases:   ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2
RefSeq:   NG_011756.1 NC_000014.8 NT_026437.12 NG_011535.1
Ensembl:   ENSG00000140009
Entrez:   2100
Chromosome Mapping:   14q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 36.620000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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