PrimePCR™ Probe Assay: KRT14, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KRT14, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KRT14, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055128
Assay Design:   exonic
Chromosome Location:   17:39738597-39738731question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000237289 ENST00000374747 ENST00000167586 ENST00000574190 ENST00000497933 ENST00000449771 ENST00000549151 ENST00000389212 ENST00000548919 ENST00000405493 ENST00000171000 ENST00000222107 ENST00000570106 ENST00000395216 ENST00000394464 ENST00000415690 ENST00000504572 ENST00000394466 ENST00000231509 ENST00000503201 ENST00000343796 ENST00000424646 ENST00000508471 ENST00000323206 ENST00000396568 ENST00000275635 ENST00000367590 ENST00000318442 ENST00000243673 ENST00000389869 ENST00000382043 ENST

Gene Information

This gene encodes a member of the keratin family the most diverse group of intermediate filaments. This gene product a type I keratin is usually found as a heterotetramer with two keratin 5 molecules a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq Jul 2008]

Gene Symbol:   KRT14
Gene Name:   keratin 14
Aliases:   CK14, EBS3, EBS4, K14, NFJ
RefSeq:   NC_000017.10 NG_008624.1 NT_010783.15
Ensembl:   ENSG00000186847
Entrez:   3861
Chromosome Mapping:   17q12-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.280000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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