PrimePCR™ Probe Assay: FLNA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FLNA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FLNA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055394
Assay Design:   exonic
Chromosome Location:   X:153582315-153582537question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000360319 ENST00000369850 ENST00000344736 ENST00000438732 ENST00000422373 ENST00000369856 ENST00000596447 ENST00000597475 ENST00000600520 ENST00000594381 ENST00000599487 ENST00000601317

Gene Information

The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins transmembrane receptor complexes and second messengers. Defects in this gene are a cause of several syndromes including periventricular nodular heterotopias (PVNH1 PVNH4) otopalatodigital syndromes (OPD1 OPD2) frontometaphyseal dysplasia (FMD) Melnick-Needles syndrome (MNS) and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Mar 2009]

Gene Symbol:   FLNA
Gene Name:   filamin A, alpha
Aliases:   ABP-280, ABPX, CVD1, DKFZp434P031, FLN, FLN-A, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, XLVD, XMVD
RefSeq:   NC_000023.10 NG_008677.1 NG_011506.1 NT_167198.1
Ensembl:   ENSG00000196924
Entrez:   2316
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996200
y-intercept 37.030000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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