PrimePCR™ Probe Assay: ACTC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ACTC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ACTC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028009
Assay Design:   Intron-spanning
Chromosome Location:   15:35082621-35083344question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000290378 ENST00000544062

Gene Information

Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms alpha beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq Jul 2008]

Gene Symbol:   ACTC1
Gene Name:   actin, alpha, cardiac muscle 1
Aliases:   ACTC, ASD5, CMD1R, CMH11, LVNC4
RefSeq:   NC_000015.9 NG_007553.1 NT_010194.17
Ensembl:   ENSG00000159251
Entrez:   70
Chromosome Mapping:   15q11-q14

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