PrimePCR™ Probe Assay: ACSL4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ACSL4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ACSL4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049533
Assay Design:   exonic
Chromosome Location:   X:108926443-108926573question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000469796 ENST00000348502 ENST00000505855 ENST00000502391 ENST00000508092 ENST00000504980 ENST00000340800

Gene Information

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity subcellular localization and tissue distribution all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq Jul 2008]

Gene Symbol:   ACSL4
Gene Name:   acyl-CoA synthetase long-chain family member 4
Aliases:   ACS4, FACL4, LACS4, MRX63, MRX68
RefSeq:   NC_000023.10 NT_011651.17 NG_008053.1
Ensembl:   ENSG00000068366
Entrez:   2182
Chromosome Mapping:   Xq22.3-q23

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