PrimePCR™ Probe Assay: PPARGC1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PPARGC1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPARGC1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026151
Assay Design:   Intron-spanning
Chromosome Location:   4:23886433-23891557question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000264867 ENST00000507380

Gene Information

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma which permits the interaction of this protein with multiple transcription factors. This protein can interact with and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure regulating cellular cholesterol homoeostasis and the development of obesity. [provided by RefSeq Jul 2008]

Gene Symbol:   PPARGC1A
Gene Name:   peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Aliases:   LEM6, PGC-1(alpha), PGC-1v, PGC1, PGC1A, PPARGC1
RefSeq:   NC_000004.11 NT_006316.16
Ensembl:   ENSG00000109819
Entrez:   10891
Chromosome Mapping:   4p15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.020000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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