PrimePCR™ Probe Assay: PPP1R12A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PPP1R12A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPP1R12A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027906
Assay Design:   Intron-spanning
Chromosome Location:   12:80202265-80203635question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000450142 ENST00000437004 ENST00000546369 ENST00000550107 ENST00000553081 ENST00000547330 ENST00000547131 ENST00000261207 ENST00000546189 ENST00000360825 ENST00000341878 ENST00000312727

Gene Information

Myosin phosphatase target subunit 1 which is also called the myosin-binding subunit of myosin phosphatase is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase) which is activated by GTP. RhoA phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jan 2009]

Gene Symbol:   PPP1R12A
Gene Name:   protein phosphatase 1, regulatory (inhibitor) subunit 12A
Aliases:   MBS, MGC133042, MYPT1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000058272
Entrez:   4659
Chromosome Mapping:   12q15-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.920000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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