PrimePCR™ Probe Assay: TNNT2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TNNT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TNNT2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029436
Assay Design:   Intron-spanning
Chromosome Location:   1:201330439-201331128question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000367322 ENST00000367318 ENST00000367320 ENST00000509001 ENST00000438742 ENST00000458432 ENST00000421663 ENST00000236918 ENST00000367317 ENST00000367315 ENST00000360372 ENST00000367319 ENST00000357848

Gene Information

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms however the full-length nature of some of these variants has not yet been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   TNNT2
Gene Name:   troponin T type 2 (cardiac)
Aliases:   CMH2, CMPD2, LVNC6, MGC3889, RCM3, TnTC, cTnT
RefSeq:   NC_000001.10 NG_007556.1 NT_004487.19
Ensembl:   ENSG00000118194
Entrez:   7139
Chromosome Mapping:   1q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.200000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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