PrimePCR™ Probe Assay: GCK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GCK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GCK, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029551
Assay Design:   Intron-spanning
Chromosome Location:   7:44187367-44189572question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000403799 ENST00000395796 ENST00000345378 ENST00000437084

Gene Information

Hexokinases phosphorylate glucose to produce glucose-6-phosphate the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM) maturity onset diabetes of the young type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq Apr 2009]

Gene Symbol:   GCK
Gene Name:   glucokinase (hexokinase 4)
Aliases:   FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2
RefSeq:   NC_000007.13 NG_008847.1 NT_007819.17
Ensembl:   ENSG00000106633
Entrez:   2645
Chromosome Mapping:   7p15.3-p15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.980000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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