PrimePCR™ Probe Assay: Bhlhe41, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Bhlhe41, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Bhlhe41, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0042540
Assay Design:   exonic
Chromosome Location:   6:145863633-145864132question
Amplicon Length:   77
Splice Variants Targeted:   ENSMUST00000032386 ENSMUST00000111703

Gene Information

This gene encodes a class E basic helix-loop-helix type transcription factor. The encoded protein is a key regulator of the mammalian circadian clock. A dominant mutation in this gene results in a short sleep phenotype. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Nov 2012]

Gene Symbol:   Bhlhe41
Gene Name:   Basic helix-loop-helix family, member e41
Aliases:   Bhlhb2l, Bhlhb3, DEC2, Sharp1
RefSeq:   NC_000072.6 NT_039360.8
Ensembl:   ENSMUSG00000030256
Entrez:   79362
Chromosome Mapping:   6 G2-G3|6 74.5 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.890000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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