PrimePCR™ Probe Assay: NRP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NRP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NRP2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024351
Assay Design:   Exonic
Chromosome Location:   2:206562340-206580932question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000360409 ENST00000450507 ENST00000417189 ENST00000357118 ENST00000357785 ENST00000412873 ENST00000272849 ENST00000540178 ENST00000540841 ENST00000355117 ENST00000340626

Gene Information

This gene encodes a member of the neuropilin family of receptor proteins. The encoded transmembrane protein binds to SEMA3C protein {sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3C} and SEMA3F protein {sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3F} and interacts with vascular endothelial growth factor (VEGF). This protein may play a role in cardiovascular development axon guidance and tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   NRP2
Gene Name:   neuropilin 2
Aliases:   MGC126574, NP2, NPN2, PRO2714, VEGF165R2
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000118257
Entrez:   8828
Chromosome Mapping:   2q33.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996400
y-intercept 34.760000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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