PrimePCR™ Probe Assay: PSENEN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PSENEN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PSENEN, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0032553
Assay Design:   Exonic
Chromosome Location:   19:36237336-36237713question
Amplicon Length:   164
Splice Variants Targeted:   ENST00000222266

Gene Information

Presenilins which are components of the gamma-secretase protein complex are required for intramembranous processing of some type I transmembrane proteins such as the Notch proteins and the beta-amyloid precursor protein. Signaling by Notch receptors mediates a wide range of developmental cell fates. Processing of the beta-amyloid precursor protein generates neurotoxic amyloid beta peptides the major component of senile plaques associated with Alzheimer's disease. This gene encodes a protein that is required for Notch pathway signaling and for the activity and accumulation of gamma-secretase. [provided by RefSeq Jul 2008]

Gene Symbol:   PSENEN
Gene Name:   presenilin enhancer 2 homolog (C. elegans)
Aliases:   MSTP064, PEN-2, PEN2
RefSeq:   NC_000019.9 NG_027934.1 NT_011109.16
Ensembl:   ENSG00000205155
Entrez:   55851
Chromosome Mapping:   19q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.800000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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