PrimePCR™ Probe Assay: EIF3C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: EIF3C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EIF3C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0039924
Assay Design:   exonic
Chromosome Location:   16:28725234-28725325question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000380876 ENST00000398944 ENST00000398943 ENST00000395587 ENST00000568426 ENST00000563139 ENST00000566866 ENST00000564243 ENST00000569690 ENST00000331666 ENST00000566501

Gene Information

Description Not Available

Gene Symbol:   EIF3C
Gene Name:   eukaryotic translation initiation factor 3, subunit C
Aliases:   EIF3CL, EIF3S8, FLJ53378, FLJ54400, FLJ54404, FLJ55450, FLJ55750, FLJ78287, MGC189737, MGC189744, eIF3-p110
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000184110
Entrez:   8663
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.950000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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