PrimePCR™ Probe Assay: CASK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CASK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CASK, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052611
Assay Design:   exonic
Chromosome Location:   X:41379713-41379832question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000421587 ENST00000378163 ENST00000378179 ENST00000378168 ENST00000378158 ENST00000378166 ENST00000442742 ENST00000318588 ENST00000361962

Gene Information

This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4 mental retardation and microcephaly with pontine and cerebellar hypoplasia and a form of X-linked mental retardation. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Mar 2010]

Gene Symbol:   CASK
Gene Name:   calcium/calmodulin-dependent serine protein kinase (MAGUK family)
Aliases:   CAGH39, CAMGUK, CMG, FGS4, FLJ22219, FLJ31914, LIN2, MICPCH, TNRC8
RefSeq:   NG_016566.1 NC_000023.10 NT_079573.4 NG_016754.1
Ensembl:   ENSG00000147044
Entrez:   8573
Chromosome Mapping:   Xp11.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.040000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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