PrimePCR™ Probe Assay: IL6ST, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IL6ST, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL6ST, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026506
Assay Design:   Intron-spanning
Chromosome Location:   5:55265634-55272119question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000381298 ENST00000381293 ENST00000522633 ENST00000336909 ENST00000381294 ENST00000542298 ENST00000381287 ENST00000536319

Gene Information

The protein encoded by this gene is a signal transducer shared by many cytokines including interleukin 6 (IL6) ciliary neurotrophic factor (CNTF) leukemia inhibitory factor (LIF) and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6 a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq Jul 2010]

Gene Symbol:   IL6ST
Gene Name:   interleukin 6 signal transducer (gp130, oncostatin M receptor)
Aliases:   CD130, CDW130, DKFZp564F053, GP130, IL-6RB
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000134352
Entrez:   3572
Chromosome Mapping:   5q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 34.750000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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