PrimePCR™ Probe Assay: NCOA2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NCOA2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NCOA2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027721
Assay Design:   Intron-spanning
Chromosome Location:   8:71044168-71050536question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000452400 ENST00000518363 ENST00000267974

Gene Information

The NCOA2 gene encodes nuclear receptor coactivator 2 which aids in the function of nuclear hormone receptors. Nuclear hormone receptors are conditional transcription factors that play important roles in various aspects of cell growth development and homeostasis by controlling expression of specific genes. Members of the nuclear hormone receptor superfamily which includes the 5 steroid receptors and class II nuclear receptors (see below) are structurally characterized by 3 distinct domains: an N-terminal transcriptional activation domain a central DNA-binding domain and a C-terminal hormone-binding domain. Before the binding of hormone steroid receptors which are sometimes called class I of the nuclear hormone receptor family remain inactive in a complex with heat-shock protein-90 (MIM 140571) and other stress family proteins. Binding of hormone induces critical conformational changes in steroid receptors that cause them to dissociate from the inhibitory complex bind as homodimers to specific DNA enhancer elements associated with target genes and modulate that gene's transcription. After binding to enhancer elements transcription factors require transcriptional coactivator proteins to mediate their stimulation of transcription initiation (Hong et al. 1997 [PubMed 9111344]).[supplied by OMIM Nov 2010]

Gene Symbol:   NCOA2
Gene Name:   nuclear receptor coactivator 2
Aliases:   GRIP1, KAT13C, MGC138808, NCoA-2, SRC2, TIF2, bHLHe75
RefSeq:   NC_000008.10 NT_008183.19
Ensembl:   ENSG00000140396
Entrez:   10499
Chromosome Mapping:   8q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.170000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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