PrimePCR™ Probe Assay: ACTN4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ACTN4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ACTN4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028372
Assay Design:   Intron-spanning
Chromosome Location:   19:39215184-39216475question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000252699 ENST00000440400 ENST00000424234 ENST00000390009

Gene Information

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions where it is involved in binding actin to the membrane. In contrast skeletal cardiac and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle alpha actinin isoform which is concentrated in the cytoplasm and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq Jul 2008]

Gene Symbol:   ACTN4
Gene Name:   actinin, alpha 4
Aliases:   ACTININ-4, DKFZp686K23158, FSGS, FSGS1
RefSeq:   NC_000019.9 NG_007082.2 NT_011109.16
Ensembl:   ENSG00000130402
Entrez:   81
Chromosome Mapping:   19q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.830000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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