PrimePCR™ Probe Assay: CSNK2B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CSNK2B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CSNK2B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040044
Assay Design:   Intron-spanning
Chromosome Location:   6:31634652-31636328question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000293829 ENST00000575331 ENST00000575398 ENST00000575082 ENST00000432290 ENST00000451917 ENST00000437010 ENST00000412802 ENST00000453234 ENST00000428483 ENST00000454382 ENST00000454511 ENST00000448596 ENST00000457089 ENST00000375885 ENST00000375882 ENST00000375865 ENST00000375866 ENST00000375880 ENST00000396170 ENST00000396167 ENST00000422261 ENST00000422567 ENST00000416194 ENST00000431476 ENST00000436169 ENST00000424502 ENST00000443673 ENST00000429633 ENST00000455161 ENST00000443952 ENST

Gene Information

This gene encodes the beta subunit of casein kinase II a ubiquitous protein kinase which regulates metabolic pathways signal transduction transcription translation and replication. The enzyme is composed of three subunits alpha alpha prime and beta which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. [provided by RefSeq Jul 2008]

Gene Symbol:   CSNK2B
Gene Name:   casein kinase 2, beta polypeptide
Aliases:   CK2B, CK2N, CSK2B, G5A, MGC138222, MGC138224
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204435
Entrez:   1460
Chromosome Mapping:   6p21-p12,6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.430000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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