PrimePCR™ Probe Assay: PRLR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PRLR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRLR, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0057883
Assay Design:   Intron-spanning
Chromosome Location:   5:35086328-35089727question
Amplicon Length:   160
Splice Variants Targeted:   ENST00000225174 ENST00000469224 ENST00000394965 ENST00000310271 ENST00000484952 ENST00000493402 ENST00000340124 ENST00000440783 ENST00000231423 ENST00000513753 ENST00000348262 ENST00000382002 ENST00000310101 ENST00000514206 ENST00000509839 ENST00000503330 ENST00000504500 ENST00000542609 ENST00000314888 ENST00000540444

Gene Information

This gene encodes a receptor for the anterior pituitary hormone prolactin and belongs to the type I cytokine receptor family. Prolactin-dependent signaling occurs as the result of ligand-induced dimerization of the prolactin receptor. Several alternatively spliced transcript variants encoding different membrane-bound and soluble isoforms have been described for this gene which may function to modulate the endocrine and autocrine effects of prolactin in normal tissue and cancer. [provided by RefSeq Feb 2011]

Gene Symbol:   PRLR
Gene Name:   prolactin receptor
Aliases:   hPRLrI
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000113494
Entrez:   5618
Chromosome Mapping:   5p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.540000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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