PrimePCR™ Probe Assay: Kpna2, Mouse

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Kpna2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Kpna2, Mouse
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qMmuCEP0043022
Assay Design:   exonic
Chromosome Location:   11:106991923-106992013question
Amplicon Length:   61
Splice Variants Targeted:   ENSMUST00000086423 ENSMUST00000022048 ENSMUST00000095724 ENSMUST00000146190 ENSMUST00000067821 ENSMUST00000057293 ENSMUST00000018506 ENSMUST00000140362 ENSMUST00000124541 ENSMUST00000144834 ENSMUST00000091289 ENSMUST00000007249 ENSMUST00000169230 ENSMUST00000109191 ENSMUST00000099069

Gene Information

Description Not Available

Gene Symbol:   Kpna2
Gene Name:   Karyopherin (importin) alpha 2
Aliases:   2410044B12Rik, IPOA1, PTAC58, Rch1
RefSeq:   NC_000077.6 NT_096135.6
Ensembl:   ENSMUSG00000018362
Entrez:   16647
Chromosome Mapping:   11 E1|11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.800000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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