PrimePCR™ Probe Assay: IBSP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IBSP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IBSP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0024553
Assay Design:   Exonic
Chromosome Location:   4:88732947-88733064question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000226284

Gene Information

The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types including hypertrophic chondrocytes osteoblasts osteocytes and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq Jul 2008]

Gene Symbol:   IBSP
Gene Name:   integrin-binding sialoprotein
Aliases:   BNSP, BSP, BSP-II, SP-II
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000029559
Entrez:   3381
Chromosome Mapping:   4q21.1

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