PrimePCR™ Probe Assay: PSMD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PSMD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PSMD1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054264
Assay Design:   exonic
Chromosome Location:   2:231931739-231934763question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000308696 ENST00000440838 ENST00000409643 ENST00000444007 ENST00000373635

Gene Information

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base which contains 6 ATPase subunits and 2 non-ATPase subunits and a lid which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome the immunoproteasome is the processing of class I MHC peptides. This gene encodes the largest non-ATPase subunit of the 19S regulator lid which is responsible for substrate recognition and binding. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq Jul 2010]

Gene Symbol:   PSMD1
Gene Name:   proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
Aliases:   MGC133040, MGC133041, P112, Rpn2, S1
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000173692
Entrez:   5707
Chromosome Mapping:   2q37.1

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