PrimePCR™ Probe Assay: LIPA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LIPA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LIPA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029565
Assay Design:   Intron-spanning
Chromosome Location:   10:90988093-91005522question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000336233 ENST00000371829 ENST00000428800 ENST00000282673 ENST00000541980 ENST00000456827 ENST00000542307

Gene Information

This gene encodes lipase A the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   LIPA
Gene Name:   lipase A, lysosomal acid, cholesterol esterase
Aliases:   CESD, LAL
RefSeq:   NC_000010.10 NG_008194.1 NT_030059.13
Ensembl:   ENSG00000107798
Entrez:   3988
Chromosome Mapping:   10q23.2-q23.3

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