PrimePCR™ Probe Assay: NOS3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NOS3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NOS3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029673
Assay Design:   Intron-spanning
Chromosome Location:   7:150696378-150698347question
Amplicon Length:   176
Splice Variants Targeted:   ENST00000297494 ENST00000461406 ENST00000484524 ENST00000467517

Gene Information

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq May 2009]

Gene Symbol:   NOS3
Gene Name:   nitric oxide synthase 3 (endothelial cell)
Aliases:   ECNOS, eNOS
RefSeq:   NC_000007.13 NG_011992.1 NT_007914.15
Ensembl:   ENSG00000164867
Entrez:   4846
Chromosome Mapping:   7q36

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