PrimePCR™ Probe Assay: COPA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COPA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COPA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031878
Assay Design:   Intron-spanning
Chromosome Location:   1:160310034-160312972question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000368069 ENST00000241704

Gene Information

In eukaryotic cells protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP beta-COP beta-prime-COP gamma-COP delta-COP epsilon-COP and zeta-COP. The alpha-COP encoded by COPA shares high sequence similarity with RET1P the alpha subunit of the coatomer complex in yeast. Also the N-terminal 25 amino acids of alpha-COP encode the bioactive peptide xenin which stimulates exocrine pancreatic secretion and may act as a gastrointestinal hormone. Alternative splicing results in multiple splice forms encoding distinct isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   COPA
Gene Name:   coatomer protein complex, subunit alpha
Aliases:   FLJ26320, HEP-COP
RefSeq:   NC_000001.10 NG_008637.1 NG_027935.1 NT_004487.19
Ensembl:   ENSG00000122218
Entrez:   1314
Chromosome Mapping:   1q23-q25

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