PrimePCR™ Probe Assay: Gria2, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Gria2, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Gria2, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0028257
Assay Design:   exonic
Chromosome Location:   2:198995803-198995903question
Amplicon Length:   71
Splice Variants Targeted:   ENSRNOT00000007061 ENSRNOT00000067113 ENSRNOT00000039587

Gene Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene. [provided by RefSeq, Jul 2008]

Gene Symbol:   Gria2
Gene Name:   Glutamate receptor 2 isoform 1 precursor
Aliases:   Not Available
RefSeq:   NM_017261 NM_001083811
Ensembl:   ENSRNOG00000028589
Entrez:   29627
Chromosome Mapping:   2q33

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