PrimePCR™ Probe Assay: RAD23A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RAD23A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAD23A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040312
Assay Design:   exonic
Chromosome Location:   19:13063815-13063925question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000316856 ENST00000586534 ENST00000592268 ENST00000590881 ENST00000541222

Gene Information

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23 a protein involved in nucleotide excision repair (NER). This protein was shown to interact with and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG) which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain which was reported to interact with 26S proteasome as well as with ubiquitin protein ligase E6AP and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq Jul 2008]

Gene Symbol:   RAD23A
Gene Name:   RAD23 homolog A (S. cerevisiae)
Aliases:   HHR23A, MGC111083
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000179262
Entrez:   5886
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.740000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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