PrimePCR™ Probe Assay: TP53AIP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TP53AIP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TP53AIP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0040721
Assay Design:   exonic
Chromosome Location:   11:128806442-128806546question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000458238 ENST00000531399 ENST00000530777 ENST00000602346 ENST00000396153 ENST00000296474 ENST00000344206 ENST00000335621 ENST00000550422 ENST00000307885 ENST00000357869 ENST00000355059 ENST00000564784 ENST00000569035 ENST00000409858 ENST00000360271 ENST00000421262 ENST00000456252 ENST00000303088 ENST00000593273 ENST00000407360 ENST00000543652 ENST00000540981 ENST00000392324 ENST00000353145 ENST00000367255 ENST00000423061 ENST00000367253 ENST00000367248 ENST00000413186 ENST00000466159 ENST

Gene Information

This gene is specifically expressed in the thymus and encodes a protein that is localized to the mitochondrion. The expression of this gene is inducible by p53 and it is thought to play an important role in mediating p53-dependent apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   TP53AIP1
Gene Name:   tumor protein p53 regulated apoptosis inducing protein 1
Aliases:   P53AIP1
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000120471
Entrez:   63970
Chromosome Mapping:   11q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.790000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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