PrimePCR™ Probe Assay: ERG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ERG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ERG, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041582
Assay Design:   exonic
Chromosome Location:   21:39795332-39795428question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000398905 ENST00000398907 ENST00000288319 ENST00000398897 ENST00000398911 ENST00000417133 ENST00000398910 ENST00000453032 ENST00000398919 ENST00000442448 ENST00000429727

Gene Information

This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development cell proliferation differentiation angiogenesis inflammation and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium inducing vascular cell remodeling. It also regulates hematopoesis and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations resulting in different fusion gene products such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Jan 2012]

Gene Symbol:   ERG
Gene Name:   v-ets erythroblastosis virus E26 oncogene homolog (avian)
Aliases:   erg-3, p55
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000157554
Entrez:   2078
Chromosome Mapping:   21q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.180000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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